Assembling Genomes: From Reads to Read-Pairs
Explore genome assembly by understanding how short DNA reads and read-pairs provide valuable information for reconstructing genomes. Learn how read length and read-pairs impact assembly accuracy and how de Bruijn graphs help manage complex genomic repeats.
We'll cover the following...
We'll cover the following...
Previously, we described an idealized form of genome assembly in order to build up your intuition about de Bruijn graphs. In the rest of the chapter, we’ll discuss a number of practically motivated topics that will help you appreciate the advanced methods used by modern assemblers.
Reads in genomes
We’ve already mentioned that assembling reads sampled from a randomly generated text is a trivial problem since random strings are not expected to have long ...